Homogeneously Staining Regions in Direct Preparations from Human Neuroblastomas1

Homogeneously staining regions in direct preparations from human neuroblastomas.

Previous descriptions of homogeneously staining regions in human neuroblastomas have been in karyotypes obtained from established cell lines. We now report homogeneously staining regions in direct preparations from two human neuroblastomas. In one of the cases, the homogeneously staining region identified in the primary tumor was also found in metastases to bone marrow and pleural fluid. The ho...

Homogeneously Staining Regions or Double Minute Chromosomes in Leukemia (Brief Communication)

Cytogenetic evidence for gene amplification in mouse skin carcinogenesis.

Using our recently developed methodology for cytogenetic evaluation of solid tumors, we analyzed the occurrence of double minutes (DM) in chemically induced mouse skin carcinomas and papillomas. These studies revealed that DM were observed in 10% of the squamous cell carcinomas and in none of the papillomas screened. In addition, we analyzed four cell lines derived from mouse skin papillomas an...

Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.

Two human neuroendocrine tumor cell lines derived from a colon carcinoma contain either numerous double minute chromosomes (COLO 320 DM) or a homogeneously staining marker chromosome (COLO 320 HSR). We found amplification and enhanced expression of the cellular oncogene c-myc in both COLO 320 DM and HSR cells, and we were able to show that the homogeneously staining regions of the COLO 320 HSR ...

Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions.

Two human colon carcinoma cell lines derived from the same tumor specimen were characterized. The cell lines, COLO 320 and COLO 321, have amine precursor uptake and decarboxylation cell properties, such as ectopic production of norepinephrine, epinephrine, serotonin, adrenocorticotropic hormone, and parathyroid hormone. The cells were morphologically different from most colon cell lines. Double...